A student assays a newly purchased enzyme by the standard procedure. He dissolves 50mg of...

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Biology

A student assays a newly purchased enzyme by the standard procedure. He dissolves 50mg of the enzyme in 100 mL water and uses 10mL for the assay. He adds the enzyme to the substrate and collects absorbance data at specified intervals. Calculate the specific activity of the enzyme if at 5 minutes absorbance was 0.345 and at 10 min absorbance was 0.645. Standard curve equation was given as Y=0.238x. Show work to get full points

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To calculate the specific activity of the enzyme we will follow these steps 1 Determine the change in absorbance A between the two time points 5 minutes and 10 minutes 2 Use the standard curve equation to convert the change in absorbance to concentration of product formed 3 Calculate the specific activity using the amount of enzyme used and the time interval Step 1 Calculate the change in absorbance A See Answer

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