A single strand of DNA, 24 nucleotides long, with the sequence 5'-TTTCCCgggAAAgggTTTAAAggg-3' is in a test tube. (Note...

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Biology

A single strand of DNA, 24 nucleotides long, with thesequence
5'-TTTCCCgggAAAgggTTTAAAggg-3'
is in a test tube. (Note that G's are shown in lowercase, sothat your eye can better distinguish them from C's)
Other than the appropriate buffer solution, what else needs togo in the test tube to so that we end up with a piece of doublestranded DNA, 24 base pairs long, with the above sequencecomprising one of the two strands? (SELECT ALL THAT APPLY)
The primer 5'-TTTCCCg-3'
The primer 5'-CCCTTTA-3'
deoxynucleotide triphosphates
deoxynucleotide monophosphates
The primer 5'-CgggAAA-3'
The primer 5'-CCTTTCC-3'
The primer 5'-TAAAggg-3'
DNA unwindase
DNA polymerase
RNA polymerase

Answer & Explanation Solved by verified expert

3.7 Ratings (414 Votes)

We have been given a single strand of DNA 24 nucleotides long with the sequence 5TTTCCCgggAAAgggTTTAAAggg3 From this given singlestranded DNA ssDNA we need to synthesize a double stranded DNA dsDNA which is also 24 base pairs long and with the given sequence comprising one of the two strands The given ssDNA would serve as a template strand for the synthesis of a complementary strand to form a doublestranded ds DNA The reagents that need to go in the test tube other than the appropriate buffer solution and the given template are the below three The See Answer

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