A sample of a peptide of unknown sequence was treated withtrypsin; another sample of the same peptide was treated withchymotrypsin.
The sequences (N-terminal to C-terminal) of the smaller peptidesproduced by trypsin digestion were as follows:
Cys—Thr—Ala—Ser—Ile—Lys
Arg—Thr—Arg—Gln—Arg
Gln—Phe—Ala—Val—Tyr—Asn—Lys
Phe—His—Cys—Asn
The sequences of the smaller peptides produced by chymotrypsindigestion were as follows:
Asn—Lys—Cys—Thr—Ala—Ser—Ile—Lys—Phe
Arg—Thr—Arg—Gln—Arg—Gln—Phe
His—Cys—Asn
Ala—Val—Ty
