A researcher was attempting to quantify the amount of dichlorodiphenyltrichloroethane (DDT) in spinach with gas chromatography using...
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A researcher was attempting to quantify the amount ofdichlorodiphenyltrichloroethane (DDT) in spinach with gaschromatography using a chloroform internal standard. To begin, theresearcher examined a sample containing 9.65 mg/L DDT standard and2.40 mg/L chloroform as the internal standard, producing peak areasof 3589 and 6209, respectively.
Then, the researcher collected 6.53 g of spinach, homogenizedthe sample, and extracted the DDT using an established method(assume 100% extraction), producing a 3.24 ml volume of unknownsample. The researcher then prepared a sample that contained 1.00ml of the unknown sample and 1.50 ml of 12.90 mg/L chloroform,which was diluted to a final volume of 25.00 ml. The sample wasanalyzed using GCMS, producing peak areas of 8017 and 13637 for theDDT in the unknown and the chloroform, respectively.
Calculate the DDT concentration in the spinach sample. Expressthe final answer as milligrams of DDT per gram of spinach.
concentration of DDT:
A researcher was attempting to quantify the amount ofdichlorodiphenyltrichloroethane (DDT) in spinach with gaschromatography using a chloroform internal standard. To begin, theresearcher examined a sample containing 9.65 mg/L DDT standard and2.40 mg/L chloroform as the internal standard, producing peak areasof 3589 and 6209, respectively.
Then, the researcher collected 6.53 g of spinach, homogenizedthe sample, and extracted the DDT using an established method(assume 100% extraction), producing a 3.24 ml volume of unknownsample. The researcher then prepared a sample that contained 1.00ml of the unknown sample and 1.50 ml of 12.90 mg/L chloroform,which was diluted to a final volume of 25.00 ml. The sample wasanalyzed using GCMS, producing peak areas of 8017 and 13637 for theDDT in the unknown and the chloroform, respectively.
Calculate the DDT concentration in the spinach sample. Expressthe final answer as milligrams of DDT per gram of spinach.
concentration of DDT:
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