A researcher wants to clone genes using recombinant gene technology as part of a genome sequencing...

80.2K

Verified Solution

Question

Biology

  1. A researcher wants to clone genes using recombinant genetechnology as part of a genome sequencing project. There are eightsteps below. First, cross out the two steps that are notpart of this process. Then, put the remaining six steps in thecorrect order.

A Insert recombinant plasmid into bacterium.

B Cut the genome into fragments using restriction enzymes.

C Determine activity using laser.

D Use DNA Ligase.

E Make cDNA using reverse transcriptase.

F Grow bacteria and add clone to genomic library.

G Cut bacterial plasmids with restriction enzymes.

H Combine DNA fragments and cut plasmid DNA.

Answer & Explanation Solved by verified expert
4.2 Ratings (675 Votes)
The following forms the reactions1 Cut the bacterial Plasmid with a restriction enzyme forfurther reaction or cloning purpose2 Cut the genome into fragments using restriction enzyme3 Use DNA ligase4Combine DNA fragment    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students