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A protein retained on an affinity chromatography column is usually eluted off the column by (circle...

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Biology

A protein retained on an affinity chromatography column isusually eluted off the column by (circle the correct answer) A.increasing the salt concentration in the buffer B. changing thetemperature of the elution buffer. C. allowing the retained proteinto naturally come off the column after the non-specifically boundproteins have first passed through the resin. D. adding theprotein's free ligand.

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Affinity chromatography is a technique for separation of ionizing molecules based on total charge This technique is used to separation of similar types of molecules which are difficult to separate by other techniques Affinity chromatography separates these molecules by altering the pH of the buffer It is used to separate proteins peptides amino acids and    See Answer
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