A nonscientist friend of yours asks how findings in a wormorflycan be relevant to human biology. Explain to your friend theimportance of model organisms in molecular biology research.
1. Compare and contast one-dimensional SDS-PAGE andtwo-dimensional gel electrophoresis of proteins.
2. You have purified a protein. When you subject it to SDS-PAGE,two bands are seen. Provide a possible explanation and describe howyou could test your hypothesis.
3. Compare and contrast the steps in production of polyclonaland monoclonal antibodies.
4. A chromatographic column in which oligo-dT is linked to aninert substance is useful in separating eukaryotic mRNA from otherRNA molecules. On what principle does this column operate?
5. You plan to use the polymerase chain reaction to amplify partof the DNA sequence shown below, using oligonucleotide primers thatare hexamers matching the regions shown in red. (In practice,hexamers are too short for most purposes). State the sequence ofthe primer oligonucleotides that should be used, including theirpolarity (5?3?), and give the sequence of the DNA molecule thatresults from amplification.
5?-TAGGCATGCAATGGTAATTTTTCAGGAACCAGGGCCCTTAAGCCGTAGGCAT-3?3?-ATCGGTACGTTACCATTAAAAACTCCTTGGTCCCGGGAATTCGGCATCGGTA-5?
6. Complete the incomplete diagrams below to show the keystructural difference between an NTP, dNTP, and ddNTP (e.g., CTP,dCTP, ddCTP).
What do “d†and “dd†stand for?
Explain why ddNTPs are called \"chain terminators\" in DNAsequencing reactions.
