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A) David, an undergraduate research assistant in our lab, performed Western Blotting experiment and he used...

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Biology

A) David, an undergraduate research assistant in our lab,performed Western Blotting experiment and he used anti- Z proteinpolyclonal antibody raised in rabbit as a primary antibody. As asecondary antibody, he used anti-mouse HRP labeled antibody. Couldyou please tell him what is wrong with this experiment?

B) David, an undergraduate intern in our lab, forget to blockthe blot with BSA and continue to the antibody hybridization stepsand finally developed his blot. Could you please describe his blotpicture without even looking!

Answer & Explanation Solved by verified expert
4.2 Ratings (627 Votes)
Since we only answer one question at a time as per the honor code we will answer the first one Western blotting is a technique used to separate and identify protein molecules from a mixture of a protein It is done by first separating the proteins in SDSPAGE followed    See Answer
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