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A chromatin immunoprecipitation (ChIP) experiment for RNA polymerase II (RNAP2) is carried out, specifically to tell...

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Biology

A chromatin immunoprecipitation (ChIP) experiment for RNApolymerase II (RNAP2) is carried out, specifically to tell thedifference between RNAP2 bound to the promoter and RNAP2 that hasescaped the promoter but has not yet begun to fully elongate (RNAP2serine 5 phosphorylated).

Describe two issues that might come up whendesigning your experiment to obtain the type of data you desire.Also provide a solution to address these twoissues.

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