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A biochemist replaces the DNA‐binding domain of the yeast Gal4 protein with the DNA‐binding domain of...

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Biology

A biochemist replaces the DNA‐binding domain of theyeast Gal4 protein with the DNA‐binding domain of the Lac repressorand finds that the engineered protein no longer regulatestranscription of the GAL genes in yeast.

A. Draw a diagram of the different functionaldomains you would expect to find in the Gal4 protein and in theengineered protein.

B. What might be done to the DNA‐binding siterecognised by this chimeric protein to make it functional inactivating transcription of the GAL genes?

Answer & Explanation Solved by verified expert
4.2 Ratings (804 Votes)
SOLUTIONthis protein is a positive regulator for the geneexpression of the galactoseinduced genes such as GAL1 GAL2 whichcode for enzymes used to convert galactose to glucoseit recognizesa 17 base pair in the yeast Saccharomyces cerevisiae regulationrest on a dosagedependent functional interplay between thepositive regulator of GLA4 protein and negative regulator GLA80proteinwe have used this interplay to select in vitro    See Answer
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