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A 5mL blood sample is treated with trichloroacetic acid for protein precipitation. After centrifugation, the remaining...

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Chemistry

A 5mL blood sample is treated with trichloroacetic acid forprotein precipitation. After centrifugation, the remaining solutionis taken to pH 3 and extracted with two portions of 5.00 mL methylisobutyl ketone, containing APCD. The extract is aspirated directlyinto a air/acetylene flame and an absorbance of 0.502 at 283.3 nmis obtained. 5.00 mL aliquots of primary standard containing 0.400y 0.600 ppm of lead are treated the same way, and absorbances of0.396 y 0.599, respectively, are obtained. Determine theconcentration of lead in the sample (in ppm) assuming it followsBeer's law.

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3.7 Ratings (311 Votes)
According to Beerlamberts law Absorbance Molar absorption coefficent Optical path length Molar ConcentrationMolar absorption coefficient is measured inM1Cm1 Optical path legth in Cm and    See Answer
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