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A 5.00 ml sample of blood was treated with trichloroacetic acid to precipitate proteine. After centrifugation,...

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Chemistry

A 5.00 ml sample of blood was treated with trichloroacetic acidto precipitate proteine. After centrifugation, the resultingsolution was brought to a PH of 3 and was extracted with two 5-ml,portions of methyl isobutyl ketone containing the organic leadcomplexing agent APCD. The extract was aspirated directly into anair-acetylene flame yielding an absorbance of 0.444 at 283.3nm.Five-milliliter aliquots of standard solutions containing 0.250 and0.450ppm BP were treated in the same way and yielded absorbances of0.396 and 0.599. Calculate the concentration(ppm) in the sampleassuming that Beer's law is followed

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4.1 Ratings (451 Votes)
we know that according to Beer lambert s law Absrobance absrobptitvity X lenght X concentration If we will plot a graph between absrobance and concentration then    See Answer
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