8. What is a Durham tube? Why is it added to a PR carbohydrate test? Could...

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Biology

8. What is a Durham tube? Why is it added to a PR carbohydratetest? Could you obtain the same data provided by a Durham tube byremoving it from the tube and adding something else to the medium?If so, what new component would you add to the medium.

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The carbohydrate fermentation test is used to determine whetheror not bacteria can ferment a specific carbohydrate Carbohydratefermentation patterns are useful in differentiating among bacterialgroups or speciesIt tests for the presence of acid andor gas produced fromcarbohydrate fermentation Basal medium containing a singlecarbohydrate source such as Glucose Lactose Sucrose or any othercarbohydrate is used for this purpose A pH indicator such asAndrades solution Bromcresol purple BCP Bromothymol blue BTBor Phenol red is also present in the medium which will detect thethe lowering of the pH of the medium due to acid production Smallinverted tubes called Durham tube is also immersed in the medium totest for the production of the gas hydrogen or carbondioxideThe term fermentation is often used to describe the breakingdown or catabolism of a carbohydrate under anaerobic conditionsTherefore bacteria capable of fermenting a carbohydrate areusually facultative anaerobesUses of Carbohydrate Fermentation TestCarbohydrate fermentation patterns can be used to differentiateamong bacterial groups or speciesAll members of Enterobacteriaceae family areglucose fermenters they can See Answer

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