8. Here we are going to consider the differences betweenspectra and chromatograms. A list is given below of characteristicsand behaviors of UV spectra and liquid chromatography. Somecharacteristics listed apply only to spectra. Other characteristicsapply only to chromatography. And here’s the tricky bit, somecharacteristics apply to both. From the list below enter eachcharacteristic that is correct for that instrumental plot on theline associated with that instrumental plot. Be careful, thinkabout your choices, misplaced characteristics are negativepoints.
Usually has molar absorptivity values reported inhomogeneous liquid solution
In our work in this lab, the solvent system is theeluent
Spans the UV and visible regions of the electromagneticspectrum
Can be used for analytical work
Displays radiation absorption by molecules insolution
Displays the retention/separation by an appropriatestationary phase of molecules in solution
Provides information about how molecules are distributedbetween mobile and stationary phases
Can be used to identify unknown components insamples
Has time as the independent variable on the detectoroutput plot
Has wavelength as the independent variable on thedetector output plot
Has absorbance as the dependent variable on the outputplot
Is concerned with electronic transitions inmolecules
Is concerned with molecular distribution betweenphases
May show the instrument output plot at a constant flowrate
A. Liquid Chromatography plot characteristics are:_______________
B. UV spectral plot characteristics are:________________