7.You want to set up a PCR reaction.The protocol suggests a 50 µL reaction volume containing 1X buffer,200 µM dNTPs, 0.5 µM each primer (forward and reverse), 250 ngtemplate DNA, and 1 U enzyme.
You have the following reagents: 5 Xbuffer, 10 mM dNTPs, 10 µM each primer (forward and reverse), 125ng/µL template DNA, and 2 U/µL enzyme.
How many µL of water, reactionbuffer, dNTPs, each primer, template DNA and enzyme would youcombine to set up that reaction? (note that the order I’ve listedthese components is the order you’d add them to the reaction)
