6) CRISPR-Cas9 and genome editing: Briefly explain the prevailing hypothesis for the bacterial origins and function of...

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Biology

6) CRISPR-Cas9 and genome editing:

  1. Briefly explain the prevailing hypothesis for the bacterialorigins and function of CRISPR-Cas9 (prior to its use in genomeengineering).
  2. Identify and briefly explain 3 important technical issues thathave been identified as major hurdles to conducting CRISPR ongermline cells in humans (e.g., technical things we need to solvebefore it would ever be reasonable to make CRISPR babies, forexample). For this question, we can ignore the  largenumber of ethical issues, and just focus on the technicalones.

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CRISPRs Cas9 Clusters of Regularly Interspaced Short Palindromic Repeats This is a specialized region of DNA having repeated nucleotide sequences and spacers Cas9 protein binding enzyme which binds    See Answer
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