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5. Below is a table showing composition data for clove oil, obtained using two different detection...

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Biology

5. Below is a table showing composition data for cloveoil, obtained using two different detection methods (with the samesample), FID and mass spectrometry. Explain why the composition(based on relative peak areas of the GC trace) is different forthese data sets. Are either of these the “real” composition of theclove oil?

Hint: Response factor

FID

MS detector

Area

Area%

Area

Area%

Eugenol

86.78

87.73

137.95

75.84

Caryophyllene

2.03

2.05

10.26

5.64

Eugenol Acetate

10.11

10.22

33.68

18.52

Answer & Explanation Solved by verified expert
3.9 Ratings (502 Votes)
Gas chromatography is a analytical separation technique to analyse volatile substances in the gas phase There are several kind of detectors used in Gas chromatography such as mass spectrometry detectors which scans the masses continuously throughout the separation Once the sample exists the column it moves to the inlet of the mass spectrometer where it is ionized and fragmented During    See Answer
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