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40 What is the role of the RNA primer in replication a The primer provides a free 3 OH group for nucleotide addition which is required by DNA Polymerase III b The primer enables the DNA polymerase to differentiate between the leading and the lagging strand c It is necessary for DNA repair enzymes to be able to differentiate between the older template strand and the newly synthesized strand d It protects the single stranded DNA from degradation prior to replication by the DNA polymerase 41 How do eukaryotic genomes differ from prokaryotic genomes a A larger portion of eukaryotic genomes are composed of non coding DNA such as introns and pseudogenes b Eukaryotic genomes are linear prokaryotic genomes are generally circular c Eukaryotes use a telomerase to replicate the ends of their chromosomes prokaryotes do not utilize a telomerase d All of the above 42 Identify which template strand below would be acting as the template for lagging strand synthesis 5 Template Strand A Template Strand B A Template Strand A B Template Strand Replication Fork 3 43 Which of the following accurately describes the role of SeqA in replication a Seqa binds single stranded DNA that forms at the replication fork protecting it from attack by cellular nucleases b SeqA binds to hemi methylated origins of replication to prevent immediate re initiation of replication c SeqA relieves torsional strain that develops at the replication fork as the double stranded DNA molecule becomes denatured

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