4. You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to...

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Biology

4. You want to clone a 1.5 kb cDNA. Which vectors discussedwould be appropriate to use? Which would be
inappropriate? Explain your answer.
5. You want to make a genomic library with DNA fragmentsaveraging about 85 kb in length. Which vectors discussed
would be appropriate to use? Which would be inappropriate?Explain your answer.
6. Describe the process of cloning a DNA fragment into themultiple cloning site of the vector pUC18. How would you
screen for clones that contain an insert? Explain the underlyingprinciple between blue-white screening.

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The cloning vectors which are discussed Options are not mentioned in the question however I will answer the question based on the general cloning vectors and their sizes a Plasmid vector 01 to 10 kb insert b Bacterial artificial chromosome insert size 35 300 kb c Yeast artificial chromosome insert size 100 1000kb d bacteriophage 4 5 kb e cosmid insert size 30 50 kb 4 The insert size is 15 kb So the plasmid vector is most suitable for such cloning Oher vectors have See Answer

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