4. What characteristic would indicate that a topoisomeraseinhibitor could be used as an antibiotic or chemotherapeutic agent?Can you name some common topoisomerase inhibitors and explain howthese inhibitors lead to cell or bacterial death?

5. How is a chromosome different from chromatin? What can beconfusing about this terminology?

6. What noncovalent interaction is responsible for DNA bindingto histones? Explain how DNA binds to a histone.

7. Name the subunits of a histone. What is the oligomericdesignation for a histone (α, β, etc.)?

8. List the noncovalent interactions that may occur to stabilizea protein binding to DNA. What is the difference between direct andindirect H-bonding?

9. What are the major protein structural motifs that play a rolein a protein binding to DNA? • What structural motif does not bindto DNA but is commonly found in DNA binding proteins? What is thefunction of the preceding motif?

10. What are the structural characteristics of the motifs thatyou mentioned in the previous question? Which of these motifsprovides the greatest recognition of a specific sequence ofnucleotides in DNA by the protein? Why?

11. What are the typical physical-chemical characteristics of amolecule that can intercalate DNA?