4. This is a coding DNA : 5’ ATG AGT GAT CTA GAC AGA CTT...

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Medical Sciences

4. This is a coding DNA : 5’ ATG AGT GAT CTA GAC AGA CTT GCA AGCCGG …3’

(a) Predict the complementary strand and identify the strandname of it. (3' --> 5’)

(b) Predict the mRNA sequence (5' --> 3’) for the givencoding strand.

(c) Predict the amino acid sequence of the gene.

(d) Predict the amino sequence, if there is a base substitutionfrom A to T at the 16th base.

(e) Which type of mutation and protein will result from the basesubstitution?

(f) Identify the location(s) likely to be cut by the restrictionendonuclease XbaI, whose recognition site is5’…T^CTAGA…3’, and predict the digested DNA sequence (doublestranded).

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coding DNA 5 ATG AGT GAT CTA GAC AGA CTT GCA AGC CGG 3 A 3 TAC TCA CTA GAT CTG TCT GAA CGT TCG GCC5 it is the template strand B mRNA is similar to the coding strand except that T is replaced by U 5 AUG AGU GAU    See Answer
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