3) You have four tubes containing the same DNA at the sameconcentrationin each tube (pH 7.5)...

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Biology

3) You have four tubes containing thesame DNA at the sameconcentrationin each tube (pH 7.5) but underdifferent environmental conditions (listed below).Â Â TheT_Mfor this DNA molecule is 78Â°C at physiological saltconditions (~100 mM NaCl):
DNA in an aqueous solution that has been digested completely tonucleotideswith DNAse I enzyme.
DNA in an aqueous solution containing no salt and has beenheated to 78Â°C.
DNA in an aqueous solution containing physiologicalconcentrations of salt and heated to 78Â°C.
DNA in an aqueous solution containing physiologicalconcentrations of salt and is heated only to 37^oC.
Match the samples above with theircorresponding OD₂₆₀readings.Â Â Next,explain your reasoning for each of the matches youmade.Â Â (For example, â€œThe DNA in sample (a)gives the highest OD reading becauseâ€¦â€).
OD₂₆₀= 1.01 Ã
OD₂₆₀= 0.195Ã
OD₂₆₀= 0.713Ã
OD₂₆₀= 0.421Ã

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4.2 Ratings (730 Votes)

3 The melting temperature Tm is temperature at which the double stranded DNA will unwind and form a single stranded molecule When DNA is heated at or above its melting temperature under physiological conditions the DNA is denatured forming a single stranded molecule In this denatured form the nitrogenous bases will become unstacked Hence their absorbance will increase This is known as hyperchromatic effect a When DNA is completely digested with DNAse I in aqueous solution the OD260 nm is See Answer

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