3. Assume that a bacterial culture is grown on medium containing radioactive thymine until essentially all...

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Biology

3. Assume that a bacterial culture is grown on medium containingradioactive thymine until essentially all of the thymine in the DNAis radioactive. Then the bacteria with labeled T were washed andtransferred to a medium containing nonradioactive T for one roundof replication. Given the four double stranded sequences below,which one is likely to represent the replicated double strandedstructure? Note: A,C,T,G represent typical nucleotides, and 3Trepresents labeled T.)
1 2 3 4
One strand
A G ³T C
A G ³T C
A G ³T C A G ³T C
One strand A G T C
T C A G
U C ³A G ³T C A G
In PCR reactions, the only enzyme that is needed is apolymerase. Provide reasoning as to why the following essentialreplication enzymes/components are not needed:
Helicase
Topoisomerase
Ligase
Primase
Replication clamp

Answer & Explanation Solved by verified expert

3.7 Ratings (728 Votes)

HELICASE We want to go through melting and melding the DNA We cant do this with helicase because once we add it it will unzip all the strands and keep continuing so Using heat we can See Answer

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