3. A researcher has identified a new protein, protein A, which they hypothesise interacts with...
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Biology
3. A researcher has identified a new protein, protein A, which they hypothesise interacts with proteinB. To investigate this interaction, the researcher first wants to determine if the two proteinscolocalise in the cell. List the type of microscope that would be best to study this, and indicatewhat other manipulations or preparations the researcher would need to do to prepare thesample for visualisation. (3 marks)To know the protein-protein interaction, we have to label the proteins with fluorescent dyes. Weneed to excite these dyes with suitable wavelength and emitted light will be absorbed and showthe protein-protein interaction. The best microscope to study the protein-protein interaction isfluorescent microscope, and fluorescent resonance energy transfer (FRET) is a popular techniqueused for the study of protein-protein interaction.Using your answer from the previous question, give one pro and one con for using that samplepreparation approach. (2 marks)
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