29 Below is a sequence recognized by a restriction enzyme One of the DNA strands is methylated and the other is not What is the role of the Me methyl groups on the second strand GAATT CTTAAG GAATT CTTAAG a Direct a restriction enzyme to cut at that sequence b Signal that the DNA is self not foreign and thus should not be cut c Signal for the virus to insert itself into that sequence region d Signal for the virus to enter into the lytic cycle and excise itself from the host cell genome 30 Which of the following steps is unique to a virus that enters into lysogeny versus a virus that moves through only the lytic cycle a Phage DNA is injected into the bacterial cell b Many copies of phage are made that move on to infect neighboring cells c The phage DNA integrates into the bacterial chromosome becoming a prophage d The virus slowly releases viral copies from its host successfully avoiding lysing the host cell 31 CRISPR is considered an example of adaptive immunity in bacteria and archaea because it a neutralizes viral DNA by using guide RNA complementary to the invading viral DNA and a CAS protein that then cleaves the target DNA b methylates host DNA preventing the virus from integrating into the host cell chromosome c it generates antibodies that recognize and destroy invading viruses d prevents the production of cell surface receptors that allow phages to get into the cell 32 When a bacterium replicates its genome and passes the replicated information on to a new cell in the process of binary fission this is an example of a horizontal gene transfer b vertical gene transfer c parallel gene transfer d mitosis 33 This is an enzyme which is capable of inserting negative supercoils within a double stranded DNA molecule and is unique to prokaryotes making it a popular target for antibiotic treatment a Helicase c Class I Topoisomerase b Telomerase d DNA gyrase Class II Topoisomerase