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  3. 2) you have 25 ml of an atp solution. you take 30

2) You have 25 ml of an ATP solution. You take 30 µl and dilute to...

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Chemistry

2) You have 25 ml of an ATP solution. You take 30 µl and diluteto a final volume of 10 ml. The absorbance of this solution is0.432. What is the concentration of the undiluted ATP solution?(molar extinction coefficient = 15,400 M-1cm-1).

If you could upload image the solution you worked out on paper,that would be preferred.

Answer & Explanation Solved by verified expert
4.0 Ratings (743 Votes)

2)

Absorbance = molar absorptivity x concentration x path length

assuming path length = 1 cm

we get,

concentration of diluted ATP solution = absorbance/molar absorptivity

                                                            = 0.432/15,400

                                                            = 2.80 x 10^-5 M

This is the concentration of 10 ml solution containing 30 uL (0.03 ml) of orginal solution

So,

concentration of original ATP solution = 2.8 x 10^-5 M x 10 ml/0.03 ml

                                                             = 9.33 x 10^-3 M

                                                             
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