2 What enzyme breaks peroxide H O2 into oxygen 0 and water H O 3 A noncompetitive inhibitor changes the shape of so it does not fit with the substrate 4 What is the catechol oxidase inhibitor used in this experiment OBJECTIVES Understand the concepts of catalysts enzymes substrates and active sites Identify the substrate enzyme and products in each experiment Describe factors that affect enzyme activity Discuss the action of the enzymes catalase amylase and catechol oxidase Compare and identify competitive and noncompetitive inhibitors Explain how the activity of catalase and catechol oxidase are measured in the experiments Design and perform an experiment to investigate the effect of temperature pH or concentration of an enzyme on the activity of catechol oxidase INTRODUCTION A catalyst is a substance that changes the rate of a chemical reaction but is not used up by t reaction Biological catalysts called enzymes change the rate of biological reactions They a required for all reactions within the human body Most enzymes are protein molecules although studies indicate that some are RNA molecule Every protein enzyme has a specific shape determined by the way the protein chain folds upc itself This folding is due to weak hydrogen bonds hotwon