2) Additional experiments confirmed that the difference in sequence between the expected genomic sequence and the experimentally...

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Biology

2) Additional experiments confirmed that the difference insequence between the expected genomic sequence and theexperimentally determined cDNA sequence is neither due to areplication error nor to a DNA sequencing mistake. One of yourcoworkers decided to perform 5 independent experiments. In eachexperiment, the gene was first transcribed and then the transcriptwas purified and reverse- transcribed to produce double strandedcDNA copies of the gene. Finally, one copy of synthesized cDNA fromeach independent experiment was sequenced (5 samples sequenced intotal). The difference between the cDNA and genomic DNA sequencesdescribed in part 1 was found in the 5 sequences. Explain why thisresult suggested that a transcription error was unlikelyresponsible for the sequence difference.

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Any genomic sequence of any gene consists of exons and introns Exon are sequences that codes for protein whereas introns are sequences present in between exons that dont code for protein When mRNA is transcribed from genomic See Answer

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