2. (3pts) You are studying a single-pass transmembrane proteinwhich is destined to the plasma membrane. The protein has a lysineamino acid which flanks the N-terminal side of the transmembranedomain and a glutamic acid amino acid which flanks the C-terminalside of the transmembrane domain. Both the N-terminus and theCterminus of the protein carries amino acid sequences that can byglycosylated.
A) (0.5pt) When the protein reaches the plasma membrane, willthe N-terminus or the C-terminus point to the extracellularside?
B) (0.5pt) When the protein reaches the plasma membrane, willthe N-terminus or the C-terminus be glycosylated?
C) (0.5pt) In a mutant protein, the flanking “K†and “E†aminoacids are switched with each other. After the protein istransported to the plasma membrane, will the Nterminus or theC-terminus point to the cytosolic side?
D) (0.5pt) When the protein is glycosylated in the ER, will theglycosylation occur in the lumen side or cytosolic side of theprotein?
E) (1pt) How does glycosylation contribute to proper proteinfolding and quality control in the ER? (5 sentence max)
