1)The transposon Tn5 was used to create a library of 50,000Escherichia coli mutant strains having random Tn5 insertions intheir chromosomal genes. A suicide delivery plasmid vector thatcarries Tn5 was used for this purpose. Explain how and why asuicide delivery plasmid was used to generate this library and giveone (1) example of how you would screen this library to findmutants with Tn5 insertions in specific genes of interest, forexample, in the lac (lactose) operon.
2) Describe the experiments by Robert Koch that underpinKoch’s postulates. What are FOUR (4) reasons why thesepostulates could be too restrictive?
