1.The procedure used to align many sequences at the same time iscalled: *

• diversity sequence alignment
• multiple sequence alignment
• local alignment
• multiple statistical alignment
• substitution matrix alignment

2.The method of alignment that tries to align regions with highlevel matches without considering the alignment of the rest of thesequence is called: *

• global alignment
• multiple sequence alignment
• local alignment
• multiple statistical alignment
• high level matching alignment

3.The type of sequence alignments that is most suited forfinding conserved patterns in DNA or protein sequences is called:*

• global alignment / multiple sequence alignment
• local multiple alignment
• local alignment / multiple polymorphism search
• multiple statistical alignment
• high level matching alignment

4. An outbreak of pneumonia has occurred. This outbreak appearsto involve a family who had attended a family gathering and weddingceremony. The samples from the patients were then tested by PCRusing the primers for 16S rRNA and RT-PCR using primers for SARSand SARS-CoV-2 coronaviruses. Select statements that are TRUEpertaining to the case involving this family. The sequence of theamplified fragment from the PCR is provided here:AACACGGAGAGTTTGATCCTGGCTCAGAACTAACGCTGGCGGCGCGTCTTAAACATGCAAGTCAAGCGGAGTAGCAATACTCAGCGGCGAACGGGTGAGTAACACGTGGGTAATCTTCCTCTGAGTCTGGGATAACTTTCCGAAAGGGAAGCTAATACTGGATGGTCCCGAGAGATCACAAGATTTTTCGGGTAAAGATTTATTGCTCGGAGATGAGCCCGCGTCCGATTAGCTAGTTGGTGAGGTAAAGGCTCACCAAGGCGACGATCGGTAGCCGGCCTGAGAGGGTGTTCGGCCACAATGGAACTGAGACACGGTCCATACTCCTACGGGAGGCAGCAGTTAAGAATCTTGCTCAATGGGGGGAACCTGAAGCAGCGACGCCGCGTGAACGATGAAGGTCTTCGGATTGTAAAGTTCAGTAAGCAGGGAAAAATAAGCAGCAATGTGATGATGGTACCTGCCTAAAGCCACCGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTATGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGGGTGCGTAGGCGGACATGTAAGTCAGGTGTGAAAACTGCGGGCTCAACTCGCAGCCTGCACTTGAAACTATGTGTCTGGAGTTTGGGAGAGGCAAGTGGAATTCCAGGTGTAGCGGTGAAATGCGTAGATATCTGGAGGAACACCAGTGGCGAAGGCGACTTGCTGGCCTAAAACTGACCCTGAGGCACGAAAGCGTGGGTAGTGAACGGGATTAGATACCCCGGTAATCCACGCCCTAAACGTTGTCTACCAGTTGTTGGGGGTTTTAACCCTCAGTAACGAACCTAACGGATTAAGTAGACCGCCTGGGGACTATGCTCGCAAGAGTGAAACTCAAAGGAATTGACGGGGGTCCGCACAAGCGGTGGAGCATGGTGGTTTAATTCGATGATACGCGAAAAACCTCACCTAGGCTTGACATGGAGTGGAATCATGTAGAGATACATGAGCCTTCGGGCCGCTTCACAGTTGCTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCACCTTATGTTGCCATCATTCAGTTGGGCACTCGTAAGGAACTGCCGGTGACAAACCGGAGGAAGGCGGGGATGACGTCAAATCCTCATGGCCTTTATGTCTAGGGCAACACACG*

• The pathogen is a type of coronavirus.
• The physician can inform the family that they are sufferingfrom a disease called leptospirosis.
• The patients should be quarantined because they haveCOVID-19
• The pneumonia is due to a bacterial infection.
• The pneumonia can be treated with antibiotics.

5.Your research group has just completed the genome sequencingfor a new bacterial species named V. novela. This newly sequencedgenome has not yet been annotated. Your colleague has been studyingthe VTOX protein in E. modeli for many years and is interested infinding out whether a homolog for the VTOX protein can be found inthe bacterial species V. novela. Select the options that are TRUEthat can be used to provide an answer to your colleague.

• Do a blastn against the PDB database.
• Use the blastp program with the VTOX protein as the query tosearch the UniProt database.
• Use the VTOX protein sequence as a query for the tblastnprogram to search the V. novela genome as the database.
• Search the E. modeli database using tblastn and the VTOXprotein as the query.
• Carry out a gene prediction for the V. novela genome and usethe resulting genes as blastx queries to search a protein sequencedatabase.