1.The following data were obtained in a study of an enzyme knownto follow Michaelis-Menten kinetics:

2/27/ 20

V0 (mol/min)

  217  325  433  488  647

Substrate added (mmol/L)

0.8 2 4 6 1,000

      

The Km for
A) 1 mM., B) 1000mM, C) 2mM, D ) 4mM, E) 6mM

this enzyme is approximately:

2. To

1. A) the enzyme concentration.

2. B) the initial velocity of the catalyzed reaction at [S]>> Km.

3. C) the initial velocity of the catalyzed reaction at low[S].

4. D) the Km for the substrate.

5. E) both the enzyme concentration and the initial velocity of thecatalyzed reaction at

   [S] >> Km.

3. An enzyme that can convert glucose into fructose is a memberof which class of enzymes?

A) Oxidoreductases, B)Transferase, C) Hydrolases, D) Lyases, E)Isomerases

4. Which amino acid is NOT capable using its side chain (Rgroup) to participate in general acid-base catalysis?
A) Asp, B) His, C) Ser, D) Val, E) Lys

calculate the turnover number of an enzyme, you need toknow:

5. Treatment of methanol poisoning by using ethanol is anexample of what type of enzyme inhibition?
A) mixed inhibition, B) uncompetitive inhibition

C) noncompetitive inhibition D) Competitive inhibition, E)Suicide Inhibition

6. What functional groups are present on this molecule?

A) ether and aldehyde, B) Hydroxyl and Aldehyde, C) Hydroxyl andCarboxylic acid, D) hydroxyl and Ester , E) hydroxyl and ketone

7. Which statement about intrinsically disordered proteins isTRUE?

A) B) C)

D) E)

They contain small hydrophobic cores.
They represent misfolded conformations of cellular proteins.
They have no stable three-dimensional structure and therefore haveno cellular function.
They are responsible for proteostasis.
They can interact with multiple protein-binding partners and arecentral to protein interaction networks.

8.
protein aggregate?

Which disease is NOT one characterized by or associated with anunfolded

A) Alzheimer disease, B) Diabetes, C) Parkinson Disuease
D) Scurvy, E) All of these diseases are linked to unfolded proteinaggregates

9. Which amino acid when repeated six to ten times at the N- orC-terminal ends of a protein allows that protein to bind to Ni2+ions?

a.Glu, b. His, c. Ala, d. Tyr, e. Asp

10. The biochemical activity of a protein, such as its enzymaticactivity, is called its _____ function.

a. phenotypic , b. genotypic, c. cellular, d. molecular, e.organismal