1.In terms of primer design, what can be done (at least in thecase of most genes) to ensure that any amplification ofcontaminating genomic DNA is not mistaken for amplification of cDNAin RT-PCR reactions? Explain why this works.
b.What properties make actin a suitable housekeeping gene forexpression analysis? What is another example of a housekeepinggene?
c.Eukaryotic cells contain several classes of RNA, of whichmessenger RNA (mRNA) is just one. What are these classes of RNA andwhat proportion of the total RNA does each of them typicallycomprise?
2.. From what source were reverse transcriptases originallyobtained from ? (now they are made recombinantly)? Briefly describethe process of reverse transcription. Â
