1.describe southern and northern hybridization and the factors that influence it? 2.describe the clinical manifestations and pathogenesis...

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Biology

1.describe southern and northern hybridization and the factors thatinfluence it? 2.describe the clinical manifestations andpathogenesis of Staphylococcu aureus infections. How are theytreated? 3.what is antiserum therapy, when is it used and whatproblems might arise from its use

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1 The hybridization of a radioactive probe to filterbound DNA or RNA is one of the most informative experiments that is performed in molecular genetics Two basic types of hybridizations are possible Southern hybridization hybridization of a probe to filterbound DNA the DNA is typically transferred to the filter from a gel Northern hybridization hybridization of a probe to filter bound RNA the RNA is typically transferred to the filter from a gel Probes are the primary tool used to identify complementary sequences of interest Generally the probe is a clone developed by inserting DNA into a vector Most often these are plasmid clones Probe a singlestranded nucleic acid that has been radiolabelled and is used to identify a complementary nucleic acid sequence that is membrane bound Factors influencing Nucleic acid hybridization Factors influencing sensitivity The sensitivity of hybridization analysis is determined by how many labeled probe molecules attach to the target DNA The greater the number of labeled probe molecules that anneal the greater is the intensity of the hybridization signal The specific activity is a measure of the incorporation of labeled nucleotidesprobe Modern labeling procedures as nick translation random priming PCR or in vitro RNA synthesis routinely provide probes with high specific activity The amount of target DNA is also See Answer

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