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1a) What are the differences between biological replicates and technical replicates? Explain. What are the appropriate applications...

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Biology

1a) What are the differences between biological replicates andtechnical replicates? Explain. What are the appropriateapplications for each?

1b) Explain why statistical analysis of whole genome expressiondata (e.g., fpkm or tpkm from RNA-seq data) require using differentcriteria for statistical significance between experimental andcontrol samples than analyzing differences in expression of asingle gene (e.g., ΔCt or ΔΔCt values fromRT-qPCR) ?

1c) How have biologists addressed the concerns of part b?

Answer & Explanation Solved by verified expert
3.8 Ratings (396 Votes)
1a Biological replicate means samples from parallel experiments whereas technical replicate means samples from the same experiment to show the variation if any and these replicates are necessary for the statistical analysis to    See Answer
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