19. Pick the correct answer.

When a gene of interest has been inserted into a plasmidvector, the result is called _____ DNA.
	homologous analogous recombinant heterologous restriction fragment

	Restriction sites are often DNA _____, which means that bothDNA strands constituting the restriction site have the same DNAsequence but in _____ orientation.
	donors; parallel palindromes; parallel palindromes; antiparallel vectors; antiparallel hybrids; parallel

The restriction enzyme XYZ-1 cuts DNA in one strand of adouble-stranded DNA molecule at the palindromic sequence5′-GAATTC-3′. What is the sequence of the opposite strand alsocleaved by this restriction enzyme?

3′-GAATTC-5′ 3′-CTTAAG-5′ 3′-GTTAAC-5′ 3′-CAATTG-5′ 3′-GAAAAG-5′ Name the key function of mitosis. to replicate DNA within a cell to generate two daughter cells genetically identical to theparent cell to generate two daughter cells that through recombination aregenetically similar to (but not identical) to the parent cell to manufacture protein to condense the DNA Name two key functions of meiosis. double the DNA content per cell in the production of gametesand properly segregate the chromosomes into each of the four spermor egg cells formed per parent cell halve the DNA content per cell in the production of gametes andproperly segregate the chromosomes into each of the four sperm oregg cells formed per parent cell halve the DNA content per cell in the production of somaticcells and properly segregate the chromosomes into each of the twodaughter cells formed per parent cell halve the RNA content per cell in the production of gametes andpermit crossing over to occur double the DNA content per cell in the production of gametesand permit crossing over to occur In an attempt to simplify meiosis for the benefit of students,mad scientists develop a way of preventing premeiotic S phase andmaking do with having just one division, including pairing,crossing over, and segregation. Would the products of such a systemdiffer from those of typical meiosis? Why? The products would be identical to typical meiosis. It would not work because the DNA needs to replicate in orderfor meiosis to function properly. Such a system would generate two cells instead of four. Such a system would not be able to provide genetically diversegametes. Human cells normally have 46 chromosomes. Let us define thetransition from one cell to two cells as mitosis proceeds. What isthe number of nuclear DNA molecules present in a human cell inmetaphase of mitosis? 92 DNA molecules per cell. Found as 92 physically separatechromosomes. 92 DNA molecules per cell. Found as 46 chromosomes each withtwo chromatids attached at the centromere. 46 DNA molecules per cell. Found as 23 chromosomes, each withtwo chromatids attached at the centromere. 23 DNA molecules per cell. Found as 23 chromosomes in each oftwo nuclei. 23 DNA molecules per cell or a haploid genetic composition. Four of the following events are part of both meiosis andmitosis, but only one is meiotic. Which one? chromatid formation chromosome movement to poles chromosome condensation synapsis spindle formation Human cells normally have 46 chromosomes. Let us define thetransition from one cell to two cells as meiosis I proceeds. Whatis the number of nuclear DNA molecules present in the human cellsformed at the completion of meiosis I? 46 DNA molecules per cell. Found as 46 physically separatechromosomes in each new cell. 92 DNA molecules per cell. Found as 46 chromosomes each withtwo chromatids attached at the centromere. 46 DNA molecules per cell. Found as 23 chromosomes, each withtwo chromatids attached at the centromere. 23 DNA molecules per cell. Found as 23 chromosomes in eachnewly formed cell. The newly formed cells will have variable numbers ofchromosomes due to genetic recombination.