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11. What are the functions of the 5’ cap? How is it added on the RNA? 12.Explain...

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Biology

11. What are the functions of the 5’ cap? How is it added on theRNA?

12.Explain how a poly-A tail is produced; (CPSF and CstF, PolyAPolymerase, Poly A Binding Protein (PABP)).

13.What are the two hypotheses for eukaryotic transcriptiontermination?

14.How does splicing of pre-mRNA occur?

15.Compare and contrast splicing of group I introns, group IIintrons and spliceosome-mediated splicing of mRNA.

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3.8 Ratings (625 Votes)
11 Functions of 5 Cap a Protection of mRNA from degradation b transport of the mRNA from nucleus to cytoplasm c binding of ribosome with mRNA Euaryotic mRNA has a peculiar enzyatically appended cap structure consisting of 7methylguanosine residue joined via 55 triphosphate bridge During Transcription 7mG is added to 5 end of nascent mRNA The initial steps in RNA capping are catalyzed by a dimeric capping enzyme which associates with the phosphorylated carboxy terminal tail domain of RNA pol II One subunit of the capping enzyme removes gamma phosphate from 5 end of the nasecnt RNA emerging from the surface of an RNA pol II The other subunut transfers the GMP moiety from the GTP to the 5dipohosphate of the nascent transcript creating the guanosine 55 triphosphate    See Answer
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