11. Sometimes the strategy for the expression of a target protein in a host organism involves...

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Biology

11. Sometimes the strategy for the expressionof a target protein in a host organism involves synthesizing theprotein as part of a fusion protein. Why is this approach useful?How is a fusion protein created?
14. A specific target DNA fragment to beintegrated into the chromosomal DNA of the host organism caninclude (1) only the target gene sequence or (2) the entireplasmid, including the target sequence. Explain how each of theseresults might occur. What are the advantages or disadvantages ofthe plasmid vector becoming incorporated into the host chromosomalDNA?

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11 proteins are widely used as therapeutics such as insulin antibioticsThese proteins are secretory and current recombinant technolgy research still suffers challenges for efficient expression and downstreamingForeign protein most of the time can undergo proteolysis if the protein is expressed in cytosolSo using fusion tags for target protein can improve the yield and solubility of their fusion partnersThe mechanism of action can be hypothesized as afusion of a stable or conserved structure to an insoluble recombinant protein may serve to stabilize and promote proper folding of the recombinant protein bFusion tags may act as a nucleus of folding molten globule hypothesis Advantages of fusion proteins technology Catalytic See Answer

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