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11. Location of a Membrane Protein The following observa- tions are made on an unknown...

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11. Location of a Membrane Protein The following observa- tions are made on an unknown membrane protein, X. It can be extracted from disrupted erythrocyte membranes into a concen- trated salt solution, and it can be cleaved into fragments by proteolytic enzymes. Treatment of erythrocytes with proteolytic enzymes followed by disruption and extraction of membrane components yields intact X. However, treatment of erythrocyte "ghosts" (which consist of just plasma membranes, produced by disrupting the cells and washing out the hemoglobin) with prote- olytic enzymes followed by disruption and extraction yields ex- tensively fragmented X. What do these observations indicate about the location of X in the plasma membrane? Do the proper- ties of X resemble those of an integral or peripheral membrane protein?

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