1) You have extracted your targeted plasmid from Milariuza. equilirus in your home. Because you do...

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Biology

1) You have extracted your targeted plasmid from Milariuza.equilirus in your home. Because you do not have an expensivenanodrop machine, you decided to run a gel to determine theconcentration of your sample. However, you also donâ€™t haveTris-acetate, thus canâ€™t make any TAE buffer. Being a smartstudent, you realize you can simply make 1X TBE buffer instead of1X TAE buffer for your experiment. You will need 800ml TBE bufferfor both making the gel and use it as a running buffer. You do nothave any access to pipettes because you left them at school, andyou only have large graduate cylinders. This means that anymeasurement below 10ml is inaccurate. The balance you have can onlymeasure accurately above 100mg. Please show all works.
Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Recipe for 5X TBE bufferÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Stock Materials
500mMTris-BorateÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 50M Tris-Borate
10mMEDTAÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â EDTA (MW ? 300)
5%NaOHÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â NaOH (MW ? 40)

Answer & Explanation Solved by verified expert

3.9 Ratings (729 Votes)

In order to make 800 ml of 1 X TBE buffer from 5 X TAE following volume is required 5 X Y 1X 800 Y 1X 800 5X Y 160 ml of 5 X TBE required For this purpose we will make 200 ml of TBE and further dilute it As give 5 X TBE 500 mM or 05 M See Answer

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