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  3. 1. you are working with chymotrypsin, and you muta

1. You are working with chymotrypsin, and you mutate the Ser-195 to Alanine and see a...

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Chemistry

1. You are working with chymotrypsin, and you mutate the Ser-195to Alanine and see a 106–fold decrease in activity. You also,separately, mutate His-57 to Alanine and see a similar decrease inactivity.

a. Draw the original, whole chymotrypsin mechanism concerningthe wild type catalytic triad. Make sure to label the the N and Cterminal ends of the peptide being cleaved, as well as the new Nand C termini formed.

b. Draw the catalytic triad with the S195A mutation, and thenthe catalytic triad with the H57A mutation, instead. Explain whichinteractions are lost, compared to the original catalytic triad. Anew student in your lab creates a chymotrypsin double mutant, withboth Ser and His being mutated to Ala at the same time. The studentsays that since the activity, individually, decreases 106 fold,together, it should decrease activity 1012-fold.

c. Is this conclusion correct? Why or why not?

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