1) You are performing an endospore stain of a 72 hour Clostridium sporogenes culture. You forget...

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Biology

1) You are performing an endospore stain of a 72 hourClostridium sporogenes culture. You forget to turn on the heat onyour water beaker. What will you see under the microscope:
a) Spores will be green
b)Everything will be green
c) vegetative cells will be pink
e) everything will be pink
2) You are performing an acid-fast stain of Mycobacteriumsmegmatis and forget to use the acid alcohol. WHat color will thecells appear under the microscope?
a)green or blue
b) purple
c) pink
d)colorless
3) You are performing a capsule stain and only see a darkbackground with no white spaces. Why?
a)the cell does not have a capsule
b) the bacteria was not added
c) crystal violet was not added
d) two of the choices are possible
4) What is NOT an advantage of having a capsule?
a) to avoid drying out
b) to avoid being eaten via phagocytosis
c) to use as food
d) to survive unfavorable conditions
5) Which of the following pairs is mismatched:
a) Carbolfuchsin: primary stain
b) alcohol: mordant
c) brilliant green: secondary stain
d) Carbolfuchsin: basic dye

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3.7 Ratings (538 Votes)

Q1 e everything will be pink Explanation Heat fixes the bacteria to the glass slide It will also helps to fix primary stain malachite green on endospore If heating will not be proper then endospore and the primary stain will be removed while washing step and everything will appear as pink due to addition of counter stain Explanation for wrong answers A and B Endospore and the primary stain will be removed during See Answer

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