1) You are a research scientist studying a novel enzyme X, and you want to characterize...

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Chemistry

1) You are a research scientist studying a novel enzymeX, and you want to characterize this new enzyme. You measure thevelocity of the reaction with different substrate concentrationsand get the following data:
[S] (mM) Initial Velocity (mmol/min)
3.0 10.4
5.0 14.5
10.0 22.5
30.0 33.8
90.0 40.5
a) Graph the above data. From the graph, estimate KMand Vmax (Michaelis-Menten Plot)
b) Use Lineweaver-Burk plot to calculate Km and Vmax.Show all equations and calculations.
c) You decide to do this experiment again, but thistime with only one third of the enzyme Xconcentration used in the first experiment. Draw a new graph on thesame graph that you did the first graph on. Estimate Km and Vmaxfrom the new graph.

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3.8 Ratings (631 Votes)

1 Plot the data in excel The MichelisMenten equation can be written as v vmaxSKm S 1 where vmax is the maximum reaction velocity maximum rate of reaction and Km is the MichelisMenten constant Plot the values S mM Initial See Answer

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[S] (mM)	Initial Velocity (mmol/min)
3.0	10.4
5.0	14.5
10.0	22.5
30.0	33.8
90.0	40.5