1. Which would have the highest melting temperature?
A. TTAACCGGGCTA
B. CATAACAAATCA
C. CGCAATAATACA
D. CGTAATAATACA
D. CGCAACCATCCG
2. Select the ones that can be applied to PCR, there can be morethan one right answer.
A. PCR can be used to obtain large quantities of a particularsequence of DNA
B. It can use short synthetic oligonucleotide primers
C. Fe2+ ions are needed for the DNA polymerase.
D. DNA polymerase can be used to synthesize DNA
E. Primers can be used and they are complementary to eachother
