1. Which would have the highest melting temperature? A. TTAACCGGGCTA B. CATAACAAATCA C. CGCAATAATACA D. CGTAATAATACA D. CGCAACCATCCG 2. Select the...

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Biology

1. Which would have the highest melting temperature?
A. TTAACCGGGCTA
B. CATAACAAATCA
C. CGCAATAATACA
D. CGTAATAATACA
D. CGCAACCATCCG
2. Select the ones that can be applied to PCR, there can be morethan one right answer.
A. PCR can be used to obtain large quantities of a particularsequence of DNA
B. It can use short synthetic oligonucleotide primers
C. Fe2+ ions are needed for the DNA polymerase.
D. DNA polymerase can be used to synthesize DNA
E. Primers can be used and they are complementary to eachother

Answer & Explanation Solved by verified expert

4.5 Ratings (875 Votes)

1 The higher the GC content of a DNA strand the higher will be its melting temperature as repeating G and C residues can form intermolecular hydrogen bonds in a single stranded DNA strand GC base pair is linked by three hydrogen bonds and is more stronger than an AT base pair which is linked by two hydrogen bonds It is calculated by the formula GC See Answer

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