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1) When 10ug of an enzyme (MW 50,000) is added to a reaction mixture containing its...

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Chemistry

1) When 10ug of an enzyme (MW 50,000) is added to a reactionmixture containing its substrate at a concentration one hundredtimes the Km, it catalyzes the conversion of 75umol of substrateinto product in 3 min. What is the enzyme's turnover number?

2) You want to load 15g of protein in 10L into one of the 12%polyacrylamide gel well. The protein needs to be in 1X buffer andin a total volume of 0.100 ml. You are given a 4.5 mg/ml proteinsolution, a 20X sample buffer, and distilled water. How much ofeach would you mix together to make required volume?

3) The Km of an enzyme of an enzyme-catalyzed reaction is 6.5uM.What substrate concentration will be required to obtain 45% of Vmaxfor this enzyme?

Answer & Explanation Solved by verified expert
3.8 Ratings (758 Votes)
Ans 1 Its assumed that at S Km velocity of enzyme catalysis is equal to its Vmax So Vmax rate of catalysis per unit time 75 umol 3 min 25 umol min1 Now using Turnover number Kcat Vmax ET    See Answer
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