1) When 10ug of an enzyme (MW 50,000) is added to a reactionmixture containing its substrate at a concentration one hundredtimes the Km, it catalyzes the conversion of 75umol of substrateinto product in 3 min. What is the enzyme's turnover number?
2) You want to load 15g of protein in 10L into one of the 12%polyacrylamide gel well. The protein needs to be in 1X buffer andin a total volume of 0.100 ml. You are given a 4.5 mg/ml proteinsolution, a 20X sample buffer, and distilled water. How much ofeach would you mix together to make required volume?
3) The Km of an enzyme of an enzyme-catalyzed reaction is 6.5uM.What substrate concentration will be required to obtain 45% of Vmaxfor this enzyme?
