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1 What is the purpose of using Ammonium Sulfate Precipitation in protein isolation and how...

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1 What is the purpose of using Ammonium Sulfate Precipitation in protein isolation and how does it work 2 Why is it necessary to perform further purification steps after ammonium sulfate precipitation in protein isolation 3 How does ion exchange chromatography contribute to the purification of proteins from spinach leaves and what property of proteins does it exploit 4 Explain the role of NaCl in ion exchange chromatography and how it helps to elute proteins from the column 5 What analytical technique is used to determine protein concentration during ion exchange chromatography and why is this important in the process

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