1. What is the importance of generating isolated bacterialcolonies?
2. Describe how a bacterial sample would be obtained from andinoculated into each of the following types of media.
Agar slant:
Agar plate:
Broth:
4. What is a subculture?
6. How would a subculture appear if a colonycontaining both S. marcescens and M. luteus wassubcultured to
a slant?
7. Condensation often gathers in the bottom ofagar slants. Why is it important in this exercise to limit
condensation on plates but not on slants?
8. Which separation method is not appropriatefor use with cultures containing a great deal of bacterial
growth? Why is this method not a good choice for theseconditions?
9. Why is agar cooled to 50°C prior to beinginoculated with bacteria? What would happen if the agar were
significantly warmer or cooler when inoculated?
10. How could you identify a potentialcontaminant on a streak plate? A pour plate? A spread plate?
11. How would any of the the isolationtechniques seen in this laboratory exercise be affected by the useof
selective medium?
