1. What is the importance of generating isolated bacterial colonies? 2. Describe how a bacterial sample would...

60.1K

Verified Solution

Question

Biology

1. What is the importance of generating isolated bacterialcolonies?
2. Describe how a bacterial sample would be obtained from andinoculated into each of the following types of media.
Agar slant:
Agar plate:
Broth:
4. What is a subculture?
6. How would a subculture appear if a colonycontaining both S. marcescens and M. luteus wassubcultured to
a slant?
7. Condensation often gathers in the bottom ofagar slants. Why is it important in this exercise to limit
condensation on plates but not on slants?
8. Which separation method is not appropriatefor use with cultures containing a great deal of bacterial
growth? Why is this method not a good choice for theseconditions?
9. Why is agar cooled to 50Â°C prior to beinginoculated with bacteria? What would happen if the agar were
significantly warmer or cooler when inoculated?
10. How could you identify a potentialcontaminant on a streak plate? A pour plate? A spread plate?
11. How would any of the the isolationtechniques seen in this laboratory exercise be affected by the useof
selective medium?

Answer & Explanation Solved by verified expert

3.6 Ratings (303 Votes)

1 Its is to get one type of culture which means one no contamination of culture with other organisms if observing through microscope one type of cell is seen it is pure culture 2 Agar slant Obtain Rotate tube around the loop Inoculate Same method Agar plate Obtain Toching the colonies using the needle Inoculate By See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member