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1. to measure DNA concentration, if you take 1/10 of a sample and read the A260...

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Biology

1. to measure DNA concentration, if you take 1/10 of asample and read the A260 and the reading is 1.5. what is the originDna concentration?

2. how to prepare a 200 ml 12% 29:1 polyacrylamidegel?

Answer & Explanation Solved by verified expert
4.3 Ratings (604 Votes)
1 DNA concentration A260 reading Path length Dilution factor 50 microgramml Path length of cuvette is usually 1 cm A260 reading 15 The sample taken was 110th However no dilution was performed on the sample Hence dilution factor is 1 DNA concentration of sample taken 151cm 1 50 microgramml 75 microgramml DNA yield microgram DNA    See Answer
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