1. This procedure recommends preparing E coli as spheroplasts to expose the alkaline phosphatase. Why is...

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Biology

1. This procedure recommends preparing E coli as spheroplasts toexpose the alkaline phosphatase. Why is this a preferred strategythan simply completely lysing the bacteria completely?
2. Explain the unit 'Unit' when attempting to express a quantityof enzyme. Relate your definiton to the reaction taking place inthe PNPP reaction.

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1 Alkaline phosphatase is one of several hydrolytic enzymes in Escherichia coli that appear to be localized in a periplasmic space that lies exterior to the bacterial cell membrane Spheroplast is a bacterium bound by its plasma membrane and the whole having a spherical form Under conditions that permitted continued protein synthesis spheroplast of Escherichia coli were unable to form active alkaline phosphatase although they synthesised protein that antigenically related to See Answer

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