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1 skt skittles Drosophila melanogaster nemo nmo The accession numbers of the reference sequence RefSeq...

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1 skt skittles Drosophila melanogaster nemo nmo The accession numbers of the reference sequence RefSeq is NM 057858 4 for the mRNA and NP 477206 1 for the protein a Add 20 nucleotides of complementary sequence to the following forward and reverse primers you do not have to take care of the reading frame at this step extra nucleotides necessary to ensure optimal restriction activity are underlined whereas restriction enzyme recognition sites are shown in bold Forward primer 5 TGTGTATGGGCCC Reverse primer 5 ATTTATGGG CCGC b What will be the theoretical length of the expected PCR product to be amplified using your section primers Be as accurate as possible when describing the expected length i e specify the exact number of nucleotides to be contained in the PCR amplicon

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